Abstract — Cervical cancer is the most common cause of women’s death after breast cancer in Indonesia, meanwhile colon cancer is the second leading cause of death from cancer among adults. One of the efforts for cancer treatment is to consume natural compounds such as Royal jelly, which has been reported to have anticancer activity. Recent studies also indicated Royal jelly action against breast cancer cell line MCF-7 1mg/mL and colon cancer cell line CaCo-2 0.5 mg/mL. This research aimed to determine cancer cell inhibition activity by Royal jelly toward cervical cell line HeLa, colon cell line WiDr and normal cell line Chang. The total phenol test, cell culture, WiDr cell preparation, Royal jelly treatments and MTT cytotoxic tests were carried out. Results showed that Royal jelly has higher inhibition activity against HeLa cell line than WiDr cell line. Royal jelly inhibited HeLa cell proliferation 36.425% and WiDr cell line 16.625% at 125μg/mL.