Abstract — Morinda officinalis How. is widely used as traditional medicinal plant with high pharmacological value. A big gap in the market for medicinal herbs in Vietnam emerged due to overexploitation and high demand for medicinal herbs. Covid-19 further boosted demand to develop medicinal plants to meet the domestic demand. There were limitations on applying fertilizers for medicinal plants in cultivation. In this study, the impact of propagation and different fertilizer applications for M. officinalis cultivation in Bac Giang Province were investigated. Data on ground diameter, height, root weight, and yield were collected after 18 and 54 months. The results showed that propagation could provide high yield and maintain good chracteristics of mother trees. M. officinalis grew well and provided high yield when it was applied with manure, mixture fertilizers and microbial fertilizers. The results of the study can be used for cultivating M. officinalis in large scale in support of the pharmaceutical industry having enough materials as well as improving livelihood of local people.